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“Whatever you can do, or dream you can, begin it. Boldness has genius, power, and magic in it.” – W. H. Murray
个人简介
党波波，陕西宝鸡人。2010年毕业于南京大学，取得理学学士学位。2015年获得芝加哥大学博士学位，期间运用蛋白质化学合成技术研究离子通道配体蛋白。2015-2019年在加州大学旧金山分校从事博士后研究，运用蛋白质设计、生物展示技术开发新型蛋白结构，发展前沿蛋白研究技术。党博士于2019年全职加入西湖大学任研究员。
学术成果
现代生物医学研究日益复杂，学科交叉往往能给生物医学研究注入新的活力，开辟新的方向。本课题组运用化学手段开发针对复杂生物体系的研究工具，并以重要蛋白为靶点开发新型蛋白质探针、大分子药物。过去的研究成果包括综合建立离子通道配体蛋白（动物毒素）化学全合成、特定位点标记的方法，用于研究离子通道在细胞内的作用机理以及动态构象变化，并以蛋白质化学合成、偶联为基础开发出新型光控神经元激活方法，为光遗传学研究提供全新的技术手段。也运用蛋白质从头设计方法开发出由共价小分子作为蛋白质核的超级稳定蛋白结构，并以此结构为基础开发蛋白质抑制剂，设计HIV疫苗。
未来课题组将围绕蛋白质工程，综合运用蛋白质化学全合成，蛋白质设计，高通量蛋白质筛选等方法一方面开发蛋白质研究的新型技术手段：包括蛋白质特定位点偶联、修饰，蛋白质高效化学切断，新型蛋白质合成方法；另一方面针对靶点蛋白，开发多肽、蛋白类大分子药物。
代表论文
1. W. Bi, # K. Tang, # G. Chen,# Y. Xie, N. F. Polizzi, W. F. DeGrado, S. Yuan,* B. Dang* An enhanced broad-spectrum peptide inhibits Omicron variants in vivo. Cell Reports Medicine, 2024, 5, 101418.
2. M. Guo,# K. Zhao,# L. Guo,# R. Zhou,# Q. He, K. Lu, T. Li, D. Liu, J. Chen, J. Tang, X. Fu, J. Zhou, B. Zheng, S. Mann, Y. Zhang, J. Huang, B. Yang, T. Zhou*, Y. Lei*, B. Dang* Copper-assisted sequence-specific chemical protein conjugation on main-chain amides. Nat. Commun., 2023, 14, 8063.
3. K. Zhao, Y. Li, M. Guo, L. Ma, B. Dang* Identification of SARS-CoV-2 PLpro and 3CLpro human proteome substrates using substrate phage display coupled with protein network analysis. J. Biol. Chem., 2023, 299, 104831.
4. M. Li,# Z. Ye,# K. Tang,# L, Guo,# W. Bi, Y. Zhang, Y. Tang, G. Rong, M. Sawan, X. Yin, R. Sun, S. Yuan,* B. Dang*. Enhanced Trimeric ACE2 Exhibits Potent Prophylactic and Therapeutic Efficacy against the SARS-CoV-2 Delta and Omicron Variants In Vivo. Cell Research, 2022, 32, 589-592.
5. W. Bi*, G. Chen, B. Dang* Novel Engineered SARS-CoV-2 HR1 Trimer Exhibits Improved Potency and Broad-spectrum Activity against SARS-CoV-2 and Its Variants. J. Virol., 2022, 96, e00681-22.
6. L. Guo,# W. Bi,# X. Wang,# W. Xu,# R. Yan,# Y. Zhang,# K. Zhao, Y. Li, M. Zhang, X. Cai, S. Jiang, Y. Xie, Q. Zhou*, L. Lu*, B. Dang*. Engineered trimeric ACE2 binds viral spike protein and locks it in “Three-up” conformation to potently inhibit SARS-CoV-2 infection. Cell Research, 2021, 31, 98–100.
7. J. Boyce, B. Dang*, B. Ary, Q. Edmondson, C. Craik, W. F. DeGrado*, I. Seiple*, “Platform to discover protease-activated antibiotics and application to siderophore–antibiotic conjugates”, J. Am. Chem. Soc., 2020, 142, 21310–21321.
8. B. Dang*, M. Mravic, B. Mensa, H. Hu, N. Schimidt, W. F. DeGrado*. SNAC-tag for sequence-specific chemical protein cleavage. Nature Methods, 2019, 16, 319–322.
9. B. Dang#, H. Wu#, V. K. Mulligan#, M. Mravic, Y. Wu, T. Lemmin, A. Ford, D. A. Silva, D. Baker, W. F. DeGrado*. De novo design of covalently constrained mesosize protein scaffolds with unique tertiary structures. Proc. Natl. Acad. Sci. USA, 2017, 114, 10852-10857.
10. B. Yang, S. Tang, C. Ma, S. T. Li, G. C. Shao, B. Dang, W. F. DeGrado, M. Q. Dong, P. G. Wang, S. Ding, L. Wang. Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions. Nat. Commun. 2017, 8, 2240.
11. T. Kubota#, B. Dang#, J. L. Carvalho-de-Souza, A. M. Correa, F. Bezanilla. Nav channel binder containing a specific conjugation-site based on a low toxicity b-scorpion toxin. Scientific Reports, 2017, 7, 16329.
12. B. Dang, R. Shen, T. Kubota, K. Mandal, F. Bezanilla, B. Roux, S. B. H. Kent. Inversion of the Side-Chain Stereochemistry of Indvidual Thr or Ile Residues in a Protein Molecule: Impact on the Folding, Stability, and Structure of the ShK Toxin. Angew. Chem. Int. Ed., 2017, 56, 3324-3328.
13. B. Dang, S. Chhabra, M. W. Pennington, R. S. Norton, S. B. H. Kent. Reinvestigation of the biological activity of D-allo-ShK protein. J. Biol. Chem. 2017, 292, 12599-12605.
14. T. Kubota, T. Durek, B. Dang, R. K. Finol-Urdaneta, D. J. Craik, S. B. H. Kent, R. J. French, F. Bezanilla, A. M. Correa. Mapping of voltage sensor positions in resting and inactivated mammalian sodium channels by LRET. Proc. Natl. Acad. Sci. USA, 2017, 114, E1857-E1865.
15. B. Dang, T. Kubota, K. Mandal, A. M. Correa, F. Bezanilla, S. B. H. Kent. Elucidation of the Covalent and Tertiary Structures of Biologically Active Ts3 Toxin. Angew. Chem. Int. Ed., 2016, 55, 8639-8642.
16. B. Dang#, B. Dhayalan#, S. B. H. Kent. Enhanced Solvation of Peptides Attached to "Solid-Phase" Resins: Straightforward Syntheses of the Elastin Sequence Pro-Gly-Val-Gly-Val-Pro-Gly-Val-Gly-Val. Org. Lett., 2015, 17, 3521-3523.
17. J. L. Carvalho-de-Souza#, J. S. Treger#, B. Dang#, S. B. H. Kent. D. R. Pepperberg, F. Bezanilla. Photosensitivity of Neurons Enabled by Cell-Targeted Gold Nanoparticles. Neuron, 2015, 86, 207-217. Highlighted in Nature, Nature Methods, Nature Review Neuroscience
18. B. Dang#, T. Kubota#, A. M. Correa, F. Bezanilla, S. B. H. Kent. Total Chemical Synthesis of Biologically Active Fluorescent Dye-Labeled Ts1 Toxin. Angew. Chem. Int. Ed., 2014, 53, 8970-8974.
19. B. Dang, T. Kubota, K. Mandal, F. Bezanilla, S. B. H. Kent. Native Chemical Ligation at Asx-Cys, Glx-Cys: Chemical Synthesis and High-Resolution X-ray Structure of ShK Toxin by Racemic Protein Crystallography. J. Am. Chem. Soc., 2013, 135,11911-11919.
联系方式
电子邮箱：dangbobo@westlake.edu.cn
实验室长期招聘分子生物学，化学方向的副研究员、博士后、研究助理，每年也通过生命科学学院生物学学科或者理学院化学学科招收博士研究生，真诚期待您的加入！
