时间：2月21日星期二16:00-17:30

Time：4:00-5:30 PM，Tuesday, Feb. 21st，2022

主持人：西湖大学生命科学学院特聘研究员 史竹兵

    Host：Dr. Zhubing Shi, PI of School of Life Sciences

    地点：云栖校区3号楼312会议室

    Venue：Room 312, Building 3, Yunqi Campus

主讲嘉宾/Speaker:

Dr. Zhucheng Chen, professor, School of Life Sciences, Tsinghua University

I am a professor of the School of Life Sciences at Tsinghua University. I received my Ph.D. in Biophysics from Cornell University and completed postdoctoral training at UTSW at Dallas. My research centers around defining the mechanisms that regulate the chromatin structure and gene transcription. My lab determined the cryoEM structures of the Snf2-like motor and the SWI/SNF family complexes bound to the nucleosome, which provide mechanistic insights into the mechanism of chromatin   

讲座摘要/Abstract:

Nucleosomes are the repeating unit of chromatin. Chromatin remodelers utilize the energy of ATP to alter the position and composition of nucleosomes, playing major roles in controlling chromatin structure and gene transcription. The SWI/SNF-family remodeling enzymes are mega-complexes assembled by the ATPase motor and a dozen of auxiliary units. Mutations of the encoding genes frequently occur in a wide spectrum of human cancers. How the remodeling complexes assemble and couple the energy of ATP hydrolysis to slide the nucleosome are long-standing questions. Here we analyze the series of cryoEM structures of the SWI/SNF-family chromatin remodeling enzymes. The ATPase motor interacts with one DNA gyre of the nucleosome, and switch the interaction register in an ATPase cycle, suggesting a “DNA wave” model of DNA translocation underlying the reaction of chromatin remodeling. The motor subunit also serves as the global organizer to orchestrate the auxiliary subunits into distinct modules for regulation and chromatin targeting. The structures have direct implications for understanding the many disease-associated mutations. Together, these findings shed mechanistic light on chromatin remodeling under health and abnormal conditions. 

联系人/Contact：

生命科学学院

于文越 yuwenyue@westlake.edu.cn