时间：5月9日星期二16:00-17:30

Time：4:00-5:30 PM，Tuesday, May 9th，2023

主持人：西湖大学生命科学学院特聘研究员 闫浈

    Host：Dr. Zhen Yan, PI of School of Life Sciences

    地点：云栖校区3号楼312会议室

    Venue：Room 312, Building 3, Yunqi Campus

主讲嘉宾/Speaker:

Sai Li, School of Life Sciences, Tsinghua University

Over the past decade, Sai Li has focused on developing cryo-ET technology and studying pathogenic enveloped viruses. His main work includes: 1) Developing high-resolution in situ structural analysis technology. He was the first to push the resolution of nuclear pore complex structures to 9Å and discovered a new luminal ring structure (Cell Res. 2020; cover story). He also resolved the S protein from the surface of SARS-CoV-2 to a resolution of 4.3Å (PNAS, 2023). He developed phase plate cryo-ET technology and resolved the glycoprotein structure from the surface of HRPV, discovering a novel membrane fusion protein with a molecular weight of 57kDa. This set a record for the smallest protein solved by phase plate cryo-ET at the time (Nat. Commun. 2019). 2) He systematically studied the in situ structures and mechanisms of assembly and infection of various enveloped viruses, including the molecular structure of intact SARS-CoV-2 (Cell 2020; ESI hot article), revealing the membrane fusion process of Rift Valley fever virus (Nat. Commun. 2018), and resolving the in situ structures of Lassa virus (PLoS Pathog. 2016) and Hantavirus (Cell Rep. 2016). His academic contributions have been widely recognized. He was invited to write reviews for journals such as Annu. Rev. Biophys. and TiBS, summarizing the cryo-ET resolution revolution and how this brought new insights into virology. He was also invited to give keynote talks at international conferences such as 3DEM Gordon Research Conference.

讲座摘要/Abstract:

We combine cryo-ET, cryo-EM, and mass-spectrometry for in situ structural characterization of emerging enveloped viruses, such as SARS-CoV-2 and influenza. The structure, landscape and site-specific glycan compositions of spike proteins were studied. We also captured snapshots of virus fusion in action, from which intermediate steps of spike-mediated membrane fusion were interpreted. We further explored how enveloped viruses prepare their spike proteins to mediate membrane fusion. The in situ observation and structural analysis have provided novel insights into the enveloped viral assembly and membrane fusion mechanism. Together, these findings have provided structural references of the antigens in situ, which helped with the development of novel vaccines.

联系人/Contact：

生命科学学院

于文越 yuwenyue@westlake.edu.cn