时间：2023年9月16日（周六）13:30-15:30

Time：1:30-3:30 PM, Saturday, September 16, 2023

地点：西湖大学云谷校区E10-222

Venue: E10-222, Yungu Campus

主持人：西湖大学生命科学学院 讲席教授裴端卿

Host: Chair Professor Duanqing Pei, School of Life Sciences

主讲人/Speaker：

Liangxue Lai

Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences

报告题目/Title:

Xenogeneic Regeneration of Humanized Mesonephros/heart in Pig

讲座摘要/Abstract:

The shortage of immunohistocompatible donors for organ transplantation is a universal problem with no direct solution. A promising alternative is to generate viable interspecies chimeras involving large mammals and human tissues through embryo complementation with pluripotent stem cells (PSCs). Pigs are a highly attractive target for growing xenogeneic human organs given their similarities with humans in body physiology and size, as well as in embryonic development. However, obtaining high degree of chimerism with species evolutionarily more distant than rodents is challenging. Although human endothelium and patches of skeletal muscle tissue have been generated inside pigs, no solid organs have been produced to date due to the overall poor contribution of human PSC-derived cells to chimeric porcine tissues. Here, to produce human cells with superior intra-niche competitiveness, we combined optimized naïve pluripotent stem cell culture conditions with the inducible over-expression of two pro-survival genes (MYCN and BCL2). The resulting cells had substantially enhanced viability in the xeno-environment of the interspecies chimeric blastocyst and successfully formed well-organized human heart and middle stage kidney until embryonic day 19 and 28 inside pig fetuses, respectively. Our findings demonstrate proof-of-principle of the possibility of generating human solid organs in organogenesis-disabled pigs, opening an exciting avenue for regenerative medicine and a unique artificial window for studying human development. 

讲座联系人/Contact:

科技合作部Sci-tech02@westlake.edu.cn