时间：11月7日星期二16:00-17:30

Time：4:00-5:30 PM，Tuesday, November 7，2023

主持人：生命科学学院特聘研究员 马丽佳

    Host：Dr. Lijia Ma, PI of School of Life Sciences

    地点：云谷校区E9-109

    Venue：E9-109, Yungu Campus

主讲嘉宾/Speaker:

Dr. Quanjiang Ji, Associate Professor, PI School of Physical Science and Technology, ShanghaiTech University

Dr. Quanjiang Ji is an Associate Professor at ShanghaiTech University and his research focuses on the mining and engineering of new CRISPR-Cas systems and the development of CRISPR-based gene therapeutics. He received his bachelor’s degree from Nanjing University in 2009, and PhD from University of Chicago in 2014. He pursued his postdoctoral training from University of California, Berkeley from 2014 to 2016. He has developed two miniature genome editing systems, CRISPR-Cas12f and -Cas12n. Moreover, he developed numerous CRISPR-based genetic manipulation methods in pathogenic bacteria, which are widely used in research labs worldwide. He serves an editor of PLoS Genetics.

讲座摘要/Abstract:

CRISPR-Cas systems have been widely harnessed for genetic engineering and gene therapeutics. However, the large sizes of the commonly used CRISPR effector nucleases (>1000 a.a.) restrict their flexibility in therapeutic applications that use the cargo-size-limited adeno-associated virus delivery vehicle. We recently developed two miniature CRISPR-Cas systems Cas12f and Cas12n (400-700 a.a.) with high efficiency and specificity. We elucidated the detailed mechanisms of why such compact systems were effective for genome editing. Using structure-guided nuclease and guide RNA engineering, we evolved Cas12f and Cas12n variants with enhanced genome editing activity. The small sizes of those nucleases offer advantages for cellular delivery, and characterizations of the nucleases will facilitate engineering more compact genome-manipulation technologies.

联系人/Contact：

生命科学学院

于文越 yuwenyue@westlake.edu.cn