时间：2022年11月7日（周一）16:00~17:30

Time：4:00-5:30 PM, Monday, November. 7, 2022

地点：云栖校区5号楼学术报告厅

Venue: Lecture Hall, Building 5, Yunqi Campus

主持人：西湖大学生命科学学院特聘研究员 周强

Host: Dr. Qiang Zhou, PI of School of Life Sciences

主讲人/Speaker：

Hong-Wei Wang

Professor of School of Life Sciences, Tsinghua University

Vice President of Tsinghua University

Dr. Wang is mainly engaged in the development and application of Cryo-EM methodologies, and is devoted to elucidating the architecture and mechanism of macromolecular complexes and assemblies with cryo-electron microscopy. So far, he has published more than 110 SCI papers in Cell, Science, Nat. Commun., JACS and other journals and has made many pioneering achievements in the development and application of cryo-EM methodology. 

报告题目/Title:

Developing Single Particle Cryo-EM towards the Understanding of RNAi Processing Machineries

讲座摘要/Abstract:

RNA interference pathway is an essential pathway in gene regulation in eukaryotic cells. Major RNase III proteins play critical roles  in the biogenesis of small RNAs. Drosha and Dicer are two RNase IIIs that are responsible to the biogenesis of miRNAs and siRNAs. While Drosha and its cofactor protein DGCR8 work together to cleave the primary miRNAs into hairpin-like pre-miRNAs, Dicer and its cofactor proteins generate double-stranded miRNAs or siRNAs by further cleaving the pre-miRNAs or dsRNAs respectively. Dr. Wang have been developing single particle cryo-EM as a major tool to dissect the structural basis of human Drosha, human Dicer and droshophila Dicer in their processing of corresponding target RNA substrates. Their structures revealed specialized substrate recognition and cleavage mechanisms among different types of the eukaryotic RNase IIIs. A comparison of these structures elucidates both novel and evolutionary conservative properties of the specific functional enzymes.

讲座联系人/Contact:

科技合作部 sci-tech02@westlake.edu.cn