时间：2023年10月31日（周二）10:00~11:30

Time：10:00-11:30 AM, Tuesday, October 31, 2023

地点：西湖大学云谷校区E10-201阶梯教室

Venue: E10-201, Yungu Campus

主持人：西湖大学生命科学学院 讲席教授付向东&助理教授万奕含

Host: Chair Professor Xiang-dong Fu & Assistant Professor Yihan Wan, School of Life Sciences

主讲人/Speaker：

John T. Lis

Barbara McClintock Professor of Molecular Biology and Genetics

Cornell University

报告题目/Title:

Mechanisms of Transcriptional Regulation of Drosophila & Mammalian Genomes

讲座摘要/Abstract:

Transcription by RNA Polymerase II (Pol II), the enzyme responsible for mRNA synthesis, is highly regulated by protein factors at multiple steps in the transcription cycle. In higher multicellular eukaryotes, Pol II pauses 20-50 bases downstream of the transcription start site on nearly all genes. This promoter-proximal pausing is an important step in transcription regulation and is dependent on several pausing factors, release factors, and elongation factors. The protein complexes DSIF (DRB Sensitivity Inducing Factor) and NELF (Negative Elongation Factor) bind to Pol II early in transcription to induce pausing. Pause release occurs upon phosphorylation of Pol II, DSIF and NELF by the Cdk9, Cyclin T1 heterodimer P-TEFb (Positive Transcription Elongation Factor b). Several elongation factors, e.g., PAF1 and Spt6, engage with Pol II in its transition from its promoter-proximal pause to productive elongation. Although Pol II pausing is a key step in transcription regulation and has been extensively studied, the molecular mechanisms of pause and pause release are far from being completely understood. Furthermore, recent studies have led to competing molecular mechanisms on the role of promoter-proximal pausing in the control of transcription. In this seminar I will describe the evolution of our understanding of transcription regulation as well as describe our latest efforts to advance and resolve this understanding using a variety of state-of-the-art genomic and optical methods in Drosophila and mammals.

讲座联系人/Contact:

科技合作部Sci-tech02@westlake.edu.cn